0055114 0008610 0006629 0006633 0005975 0014070 0008283 0050873 0006096 0016477 0045471 0010033 0006090 0009749 0006084 0019432 0006641 0005977 0006086 0009267 0016126 0006695 0033574 0009058 0042593 0007595 PC10 0006955 0043066 0006954 0030509 0034097 0030900 0009612 0016525 0030593 PC20 PC20 0051384 0048545Biological procedure 199 genes chosen Metabolic course of action Oxidation reduction Lipid biosynthetic method Lipid metabolic approach Fatty acid biosynthetic course of action Carbohydrate metabolic procedure Response to organic cyclic substance Cell proliferation Brown fat cell differentiation Glycolysis Cell migration Response to ethanol Response to organic substance Pyruvate metabolic approach Response to glucose stimulus Acetyl-CoA metabolic procedure Triglyceride biosynthetic process Triglyceride metabolic procedure Glycogen metabolic course of action Acetyl-CoA biosynthetic course of action from pyruvate Cellular response to starvation Sterol biosynthetic approach Cholesterol biosynthetic process Response to testosterone stimulus Biosynthetic course of action Glucose homeostasis Lactation 226 genes chosen Immune response Adverse regulation of apoptosis Inflammatory response BMP signaling pathway Response to cytokine stimulus Forebrain improvement Response to mechanical stimulus Negative regulation of angiogenesis Neutrophil chemotaxis 65 genes chosen 118 genes chosen Response to glucocorticoid stimulus Response to steroid hormone stimulus Glucose metabolic processRatioP values37/756 23/591 16/92 13/232 12/59 10/206 9/274 8/190 7/34 7/45 7/112 7/153 7/163 6/21 6/118 5/11 5/13 5/40 5/42 4/7 4/23 4/28 4/32 4/41 4/58 4/62 4/65 10/243 9/284 7/170 6/65 6/119 5/94 5/102 4/29 4/7.Pyraclostrobin Fungal 9E-15 8.FX1 Protocol 2E-12 five.0E-14 five.2E-09 1.2E-14 1.1E-06 eight.1E-05 3.7E-05 3.5E-09 2.3E-08 9.6E-06 six.8E-05 1.0E-04 six.9E-09 1.3E-04 1.3E-08 three.PMID:24101108 0E-08 7.1E-06 9.0E-06 1.6E-07 1.7E-05 three.6E-05 6.1E-05 1.6E-04 five.8E-04 7.4E-04 8.8E-04 1.5E-05 two.7E-04 two.8E-04 9.8E-06 two.7E-04 six.9E-04 9.9E-04 6.8E-05 1.4E-No annotation was drastically marked 5/163 4/64 4/79 four.3E-04 1.1E-04 two.6E-AGE (2013) 35:1143156 Fig. three Quantitative analysis with the mRNA expression of SREBPs, SREBP-1regulated genes, SREBP-2regulated genes, and macrophage-related genes in WdAL, WdCR, and TgAL rats. The mRNA expression levels in WAT of SREBPs (A: SREBP-1a, SREBP-1c, and SREBP-2), SREBP-1regulated genes [B: fatty acid synthase (FASN) and acyl CoA carboxylase 1 (ACC1)], SREBP-2-regulated genes [C: squalene epoxidase (Sqle) and mevalonate kinase (Mvk)], and macrophage-related genes [D monocyte chemotactic protein-1 (MCP-1), F4/80, integrin alpha X (Itgax, CD11c), and CD163] were analyzed by real-time RTPCR. Data have been normalized against TBP expression. Values are suggests EM (n0 three). *P0.05, **P0.01, and ***P0.001 vs. WdAL; # P0.05, ##P0.01, and ### P0.001 vs. WdCR (Tukey’s t test)To confirm the CR-associated SREBP-1- and macrophage-related gene expression profiles observed in wild-type rats, we analyzed the effect of CR on Tg rats. Similarly towards the wild-type rats, CR markedlyupregulated the expression of SREBP-1c, and slightly enhanced the expression of SREBP-1a (P 00.08; Fig. 4a). CR also markedly enhanced the expression of the SREBP-1-regulated genes, FASN and ACC1152 Fig. four Quantitative analysis on the mRNA expression of SREBPs, SREBP-1regulated genes, and macrophage-related genes in TgAL and TgCR rats. The mRNA expression levels in WAT of SREBPs (a SREBP1a and SREBP-1c), SREBP1-regulated genes [b fatty acid synthase (FASN) and acyl CoA carboxylase 1 (ACC1)], a.