Y expressed FLAG-tagged human RCAN1.1S protein ( 30 kDa; for much more details, see Oh et al., 2005; Hoeffer et al., 2007). Reduce blot is stained using a FLAG antibody, confirming expression of transgene. Nse-Cre driver line, CamkII -Cre (T-29) driver line. GAPDH, loading handle. Image is representative of three independent blots. WT represents the WT control for every breeding cross. F, Mice overexpressing human RCAN1 early in improvement (Nse-RCAN1Tg1a) display decreased EPM open-arm time compared with WT littermates. No difference in open-arm time was observed driving an alternative RCAN1 construct (RCAN1Tg1) together with the same Nse-Cre driver. No difference in open-arm time was also observed with postdevelopmental RCAN1 overexpression (CamkII -Cre) of TMPRSS2, Human (P.pastoris, His) either RCAN1 construct. WT-Tg1a (Nse) denotes manage RCAN1Tg1a-only (no Cre) “WT” littermates from RCAN1Tg1a Nse-Cre lines. WT-Tg1 (Nse) denotes manage RCAN1Tg1-only (no Cre) “WT” littermates from RCAN1Tg1 Nse-Cre lines. WT-Tg1 (CamkII ) denotes control RCAN1Tg1a-only (no Cre) “WT” littermates from RCAN1Tg1a CamkII -Cre lines. WT-Tg1 (CamkII ) denotes handle RCAN1Tg1-only (no Cre) “WT” littermates from RCAN1Tg1 CamkII -Cre lines. N 36 Nse-RCAN1Tg1a, 40 WT-Tg1a (Nse), 7 Nse-RCAN1Tg1, 11 WT-Tg1 (Nse), 32 CamkII -RCAN1Tg1a, 38 WT-Tg1a (CamkII ), 11 CamkII -RCAN1Tg1, 17 WT-Tg1 (CamkII ). For some datasets not ordinarily distributed, Mann hitney evaluation was made use of. p 0.05, or p 0.01, p 0.001.Hoeffer, Wong et al. ?RCAN1 Modulates Anxiousness and Responses to SSRIsJ. Neurosci., October 23, 2013 ?33(43):16930 ?6944 ?Table 1. OFA activity of mice that overexpress human RCAN1a Time/zone (s) Distance/zone (cm) Genotype Nse-RCAN1 Imply SEM WT-Tg1a (Nse) Imply SEM p value Nse-RCAN1Tg1 Imply SEM WT-Tg1 (Nse) Imply SEM p worth CamkII -RCAN1Tg1a Imply SEM WT-Tg1a (CamkII ) Imply SEM p worth CamkII -RCAN1Tg1 Mean SEM WT-Tg1 (CamkII ) Imply SEM p valueTg1aAmbulatory (s) Periphery Center Total 62.1 4.3 20.8 two.4 82.9 4.Resting (s) Periphery Center Total 580.1 13.1 69.9 ten.3 650 6.TGF beta 3/TGFB3 Protein Gene ID Vertical (s) Periphery Center Total 92 six.7 7.six 1.7 99.six 7.Stereotypic (s) Periphery Center Total 125.3 two.6 25.three three.three 150.six three.Periphery Center Periphery Center Total 767.six 15.5 132.four 15.five 1576.two 73.4 513.9 55.6 2090.1 81.779.five 120.five 1596.3 510.six 2106.9 61.7 10.eight ten.8 99.7 60.6 128.four four.9 0.542 0.542 0.87 0.968 0.911 0.952 718.five 35.eight 181.5 35.eight 1633.3 92.1 572.3 78.two 2205.six 140.9 69.8 9.20.8 82.5 594.9 2.8 6.2 12.three 0.995 0.956 0.417 19.4 three.1 89.two 11.1 549.five 29.63 658 98.4 6.8 9.5 eight.three 0.591 0.475 0.545 101.7 27.two 651.2 16.1 63.7 9.6.3 104.7 122.eight 22.4 145.2 1.2 9.1 three.2 2.1 3.9 0.533 0.669 0.538 0.476 0.284 five.5 two.five 3.72 0.7 0.495 eight.9 2.four 7 1.4 0.496 1.three 0.6 1.1 0.7 0.835 69.2 10.three 114.4 four.8 29.eight five.three 144.two 6.732.1 167.9 1551.9 623.0 2174.9 63.0 37.2 37.two 74.7 88.six 105.three 9.2 0.804 0.804 0.502 0.679 0.864 0.622 717.8 32 746.two 17.9 0.74 799.five 13.2 822.1 ten.five 0.191 182.two 32 1802.1 97.1 677.eight 75.2 2479.9 128.six 73.128.two 91.three 536.0 106.five 642.5 44.8 5.four 10.7 27.1 29.5 14.0 8.1 0.184 0.894 0.737 0.905 0.686 0.139 27.four three.1 100.four 6.3 536.9 18.four 85 17.2 621.9 9 77.4 7.48.five 115.4 35.8 151.two eight.6 7.0 7.1 three.0 0.131 0.904 0.517 0.333 86.3 8.7 131.9 four.1 31.six 3.9 163.five three.153.eight 1873.5 706.8 2580.three 75.1 17.9 89.5 61.7 120.three 4.7 0.74 0.59 0.765 0.57 0.77 one hundred.5 13.two 1736.five 123.six 357.four 43.1 2093.9 137.six 70 6.30 105 546.7 2.eight six.two 12.6 0.535 0.604 0.659 13.three 1.8 83.three 7 595 14.72.7 619.4 76.four eight.2 9 7.2 0.512 0.844 0.92 50.7 7.7 645.7 11 50.7 7.83.4 128.7 29.7 158.three eight.1 two.six two.7 3.three 0.8.