Eed oil of 57.5 CTm in the similar NUAK1 Inhibitor Gene ID temperature (Catarelli et al. 1993). It has density and precise gravity of 0.942 and 0.926 at 20 , respectively. The certain gravity of this study is lower than the distinct gravity of cashew nut (0.964) (Aremu et al., 2006) and caster seed oil (0.958) (Akpan et al. 2007). The refractive index of this study (1.473) is in close agreement with 1.465 (Aremu et al. 2006), 1.462 (Akintayo and Bayer 2002) and 1.468 (Akpan et al. 2007) of castor, akee pulp and cashew nut seed oils, respectively. This implied that brebra oil is less thicker than most of drying oils whose refractive indices range from 1.475 to 1.485 (Duel and Tr 1951). The saponification value on the oil in this study was 174.95 mg KOH/gm. This was reduced than the values for some frequent oils like castor seed oil (185.83) (Akpan et al. 2007), palm oil (196-205 mgKOH/g), groundnut oil (188-196 mgKOH/g), corn oil (187-196 mgKOH/g) as reported by Akinhanmi et al. (2008), coconut oil (253 mg KOH/gm) and palm kernel oil (247 mg KOH/ gm) (Pearson, 1976). Nevertheless, this saponification value is within exactly the same range of some edible oils reported by Eromosele and Paschal (2002). Furthermore, saponification worth (174.95) from the oil in this investigation is pretty much inside the range of (17587) ASTM (2002) specification for oils. In line with Pearson (1976), oils with decrease saponification values contain higher amount of extended chain fatty acids. Hence, the value obtained for brebra seed oil contained high quantity of higher fatty acids (as it is presented on Table 6, fatty acids 18 carbonAndualem and Gessesse SpringerPlus 2014, 3:298 http://springerplus/content/3/1/Page six ofTable 5 Brebra oil chemical and physical characteristicsOil physicochemical qualities Colour Density at 20 Precise gravity at 20 Kinematic Viscosity at 40 Acid worth pH worth Saponification value Refractive index at 40 Iodine value Peroxide worth Ester valueAll values are imply worth of triplicates.Unit Kg/m3 kg/l mm2/s mg KOH/gValues Pale-yellow color limpid liquid 0.942 0.926 40.59 0.39 6.mg KOH/g174.95 1.gI2/100 gm mEq/Kg104.48 six.88 174.chain accounts 92.eight ). The value of viscosity of brebra oil was 40.49 mm2/s (as pointed out above) is found to become significantly less than the viscosity of cashew nut oil (56 mm2/s) (Akinhanmi et al. 2008). For production of biodiesel, consequently, transesterification reaction may be the Tyk2 Inhibitor manufacturer greatest approach to lessen the viscosity from the oil. The oil under investigation has extremely low acid worth of 0.39 mg KOH/g when compared with cashew nut oil (0. 82 mg KOH/g) (Aremu et al. 2006), refined castor oil (0.869) and crude castor oil (1.148) (Akpan et al. 2007), plukenetia conophoora (11.5 mg KOH/g) as reported by Akintayo and Bayer (2002), aenniseed (47.6 ) by Ohsodi (1992). The acid value in this oil is beneath the maximum limit (2.0 mg KOH/g) of DIN EN ISO 660 and practically within the array of ASTM specification (0.four – 4.0) of castor oil (ASTM 2002).Table 6 Fatty acid composition of fatty acid of brebra oil (FAs = fatty acids)No 1 two 3 4 five Fatty acids Unsaturated FAs Oleic Linoleic Linolenic Arachidonic Eurcic Total Saturated FAs six 7 8 9 Palmitic Stearic Arachidic Behenic Total C16:0 C18:0 C20:0 C22:0 7.two 1.9 3.3 six.9 19.three C18:1 C18:two C18:three C20:4 C22:1 0.6 48.2 27.7 0.8 three.4 80.7 Carbon quantity Quantity ( )The low saponification value and acid worth imply that the oil is additional appropriate for biodiesel because the oil acid value for biodiesel, which can be significantly less than 1 . The iodine value of brebra o.