ntributions NM, GJD, HSO, and JGB designed and planned the research. MH ready fungal cultures. CB and SS ready activitybased probes made use of in this study. NM collected secretome samples and performed activitybased protein profil ing experiments. NM collected and analysed proteomic data. DN performed bioinformatic evaluation. NM and MS ready P. pastoris strains, produced and purified recombinant enzymes, and performed activity assays. NM wrote the manuscript with input from all the authors. All authors study and authorized the final manuscript. Funding The authors thank the Natural Sciences and Engineering Study Council of Canada (PostDoctoral Fellowship to NGSM), the Royal Society (Ken Murray Research Professorship to GJD), the Biotechnology and Biological Sciences Investigation Council (BBSRC) (grant BB/R001162/1 to GJD), the French GSK-3α custom synthesis National Analysis Agency (ANR13BIME0002 to JGB), the Netherlands Organization for Scientific Investigation (NWO Top rated grant 2018714.018.002 to HSO), plus the European Analysis Council (ERC2011AdG290836 “Chembiosphing” to HSO, ERC2020SyG951231 “Carbocentre” to GJD and HSO). Proteomics information have been collected at the York Centre of Excellence in Mass Spectrometry, which was designed due to a significant capital investment by means of Science City York, sup ported by Yorkshire Forward with funds in the Northern Way Initiative, and subsequent assistance from EPSRC (EP/K039660/1; EP/M028127/1). Availability of data and supplies Pichia pastoris strains and samples of recombinant proteins might be accessible from Gideon Davies ([email protected]). Samples of ABPCel, ABPXyl, and ABPGlc may perhaps be offered from Herman Overkleeft (h.s.overkleeft@lic. leidenuniv.nl). Basidiomycete fungi are available in the fungal 5-HT3 Receptor web culture collection with the International Centre of Microbial Sources (CIRMCF) at the French National Institute for Agricultural investigation (INRA; Marseille, France). Genome sequences for every single on the fungi utilised in this study are accessible from Mycocosm (mycocosm.jgi.doe.gov/mycocosm/home) (DOE Joint Genome Institute, Walnut Creek, California). Other datasets employed and/or ana lysed during the present study are available from the corresponding author on affordable request.Author details 1 York Structural Biology Laboratory, Division of Chemistry, The University of York, Heslington YO10 5DD, York, UK. two Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300 RA Leiden, The Netherlands. three UMR1163 Bio diversitet Biotechnologie Fongiques, Facultdes Sciences de Luminy, INRAE, Aix Marseille Univ, 13288 Marseille, France. 4 Polytech Marseille, Aix Marseille Univ, 13288 Marseille, France. Received: eight October 2021 Accepted: 6 JanuaryDeclarationsEthics approval and consent to participate Not applicable. Consent for publication Not applicable. Competing interests The authors declare no competing interests.References 1. Scheller HV, Ulvskov P. Hemicelluloses. Annu Rev Plant Biol. 2010;2(61):2639. 2. Luis AS, Briggs J, Zhang X, Farnell B, Ndeh D, Labourel A, et al. Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018;3(2):210. three. Celiska E, Nicaud JM, Bialas W. Hydrolytic secretome engineering in Yarrowia lipolytica for consolidated bioprocessing on polysaccharide sources: critique on starch, cellulose, xylan, and inulin. Appl Microbiol Biotechnol. 2021;105(3):9759. 4. Schlembach I, Hosseinpour Tehrani H, Blank LM, B hs J, Wierckx N, Regestein L, et al. Consolidate