The production of drug-loaded EVs and to explore possible application for in situ drug delivery technique. P2Y14 Receptor manufacturer Funding: This analysis is funded by Focused Ultrasound ROCK1 Storage & Stability Foundation.OS23.Extracellular Vesicles for new Molecular Insight to Biomolecular Interactions Tamas Beke-Somfaia, Priyanka Singhv, Imola Szigyarto and Zoltan VargacaPI, Budapest, Hungary; bMs, Budapest, Hungary; cResearch Centre for All-natural Sciences, Hungarian Academy of Sciences, Budapest, HungaryIntroduction: The prospective of extracellular vesicles (EVs) to revolutionize the diagnosis and therapy of a variety of illnesses has been realized and hence it is actually an extensively studied path. Even so, EVs are also in the size range appropriate for membrane biophysics, while they preserve the complex composition of a biological bilayer. Consequently, they may be optimal for monitoring the structure, orientation and function of biomolecules associated to EVs.Procedures: The investigated red blood cell-derived vesicles (REVs) were isolated from blood employing a typical protocol and purified employing size-exclusion chromatography. REVs had been subjected to IR, CD and flow-Linear Dichroism spectroscopy, freeze-fracture Transmission Electron Microscopy also as Dynamic Light Scattering. Benefits: Right here we demonstrate that polarized light spectroscopy techniques can deliver important information on REVs and molecules inserting into their bilayer. Flowlinear dichroism (flow-LD) measurements show that EVs can be oriented by shear force, insight into properties of oriented macromolecules in the vesicles. The Soret-band on the LD spectra demonstrates that hemoglobin molecules are oriented and connected to the lipid bilayer in freshly released REVs [1]. Further on, we selected 3 distinct antimicrobial peptides (AMPs), CM15, melittin and gramicidin and investigated their interactions with REVs applying a diverse set of methods. The peptide-membrane interactions reveal quite a few novel function of AMPs, like their ability to get rid of related proteins in the surface of REVs (Figure 1). [1] I. Cs. Szigy t R. De , J. Mih y, S. Rocha, F. Zsila, Z. Varga, T. Beke-Somfai. Flow-alignment of extracellular vesicles: structure and orientation of membrane linked biomacromolecules studied with polarized light. ChemBioChem. 2018;19:54551 Summary/Conclusion: In conclusion, EVs present great possibilities to greater realize the function and mechanism of all-natural membrane active biomolecues. Funding: This work was funded by the Momentum programme (LP2016-2), by the National Competitiveness and Excellence System (NVKP_16-1-20160007) and BIONANO_GINOP-2.3.2-15-2016-00017. The J os Bolyai Analysis Scholarship (Z.V.) is considerably acknowledged.JOURNAL OF EXTRACELLULAR VESICLESSymposium Session 24: Mechanisms of EV Delivery Chairs: Pieter Vader; Hang Hubert Yin Place: Level B1, Hall B 13:004:OS24.State with the art microscopy for live cell study with the extracellular vesicle-mediated drug delivery Ekaterina Lisitsynaa, Kaisa Rautaniemia, Heikki Saarib, Timo Laaksonena, Marjo Yliperttulab and Elina Vuorimaa-Laukkanena Laboratory of Chemistry and Bioengineering, Tampere University of Technology, Tampere, Finland; bDivision of Pharmaceutical Biosciences and Drug Research System, Faculty of Pharmacy, University of Helsinki, Helsinki, FinlandaSummary/Conclusion: This analysis offers new realtime solutions to investigate EV kinetics with living cells and complements the current techniques. The findings from the study strengthen the.