Ons produced it also attainable to characterize FAMEs with a comThe
Ons produced in addition, it doable to characterize FAMEs having a comThe its marking pheromone [41]. The of double and of B. pratorum FAMEs is crepenynic acid 5. The ester with a single bination chromatogramtriple bonds. For instance, shown in Figuremethyl MS/MS spectra The satellite fragment ions created it TB ,9c) offered a spectrum and fragdouble bond and a single triple bond six) offered abundant and recognizable with all the the majority of diunsaturated FAMEs (Figure(FAME 18:2n-6also possible to characterize FAMEs with bination of double 150.1 (Figurebonds. signal is often a diagnostic fragmentacid methyl ester w abundant peak at m/z and triple four). FAME 25:2n-7,17, and 26:2n-7,17. for triple bond ments interpreted as FAME 24:2n-7,17,This As an example, crepenynicThe double bond ( n-6TB simply because its satellite seems with previous function, ,9c) (m/z positions with the doupositions)had been and a single triple bond a 15 Da 18:2n-6TBwhere the 165.0). Analogously, double bond in outstanding agreement at(FAMEhigher m/z valueprovided a spectrum with th TB the m/z 276.1 with its satellite ble bonds had been established using m/z 291.1 isThistriple bonda diagnostic fragment for abundant peak at m/z 150.1at dimethyl disulfide derivatization [41]. peak ( n-6 ). tripl (Figure four). the signal is diagnostic Fragment m/z 190.1 indicates a double bond ( n-9) mainly because its satellite peak seems at ( n-6TB) for the reason that its satellite seems at a 15 Da greater m/z worth (m/z 165.0). Analo m/z 204.1.Figure 3. APCI MS/MS CID spectrum of [M + 55]+ adduct of stearolic acid methyl ester (FAME 18:1n9TB ); MBR APCI 192 – 349 = 79. Figure 3. = 236 + MS/MS CID spectrum of [M + 55]+adduct of stearolic acid methylester18:1n-9TB); MBR = 236 + 192 – 349 = 79.the m/z 276.1 with its satellite at m/z 291.1 will be the triple bond diagnostic peak ( Fragment m/z 190.1 indicates a double bond ( n-9) due to the fact its satellite peak app m/z 204.1. two.three. Analysis of Natural SamplesThe fragmentation of FAME standards with a variety of arrangements of double ple bonds helped us characterize the FAMEs isolated from biological samples. Th tification process was initiated by deducing the number of carbons and degree of uration in the m/z values from the protonated FAMEs. The second step examin MS/MS spectra of [M + 55]+ions to determine the diagnostic fragments and their sa The m/z values in the diagnostic fragments, MBR values, and also the mass distinction b the diagnostic fragments and satellites were used to deduce the positions of doub triple bonds. Ultimately, the retention times have been checked for their consistency with pected elution order of FAMEs [80,81].Figure four. APCI MS/MS CID spectrum with the [M + 55]+ adduct of crepenynic acid methyl ester [M + 55]+of crepenynic acid methyl ester Figure 4. APCI MS/MS CID spectrum TB 347 = 119. (FAMEFAMEs,9c); MBR = 276 + 190 — 347 = 119. 18:2n-6TB ,9c); MBR = 276 + Physique of Bombus pratorum (FAME two.three.1. 18:2n-6 from the FatThe early-nesting bumblebee Bombus pratorum is widespread in Europe. It is two.3. Evaluation of BMS-986094 Biological Activity All-natural Samples the earliest bumblebee species to emerge from Compound 48/80 Autophagy hibernation each doubleThe fat bod The fragmentation of FAME requirements with many arrangements of year. andtriple bonds helped us characterize the FAMEs isolated from biological samples. The identification procedure was initiated by deducing the amount of carbons and amount of unsaturation from the m/z values in the protonated FAMEs. The second step examined theMolecules 2021, 26,pratorum males includes TGs with long, diunsaturated fatty acyls, which ar.