T (Afghanistan, Cyprus, Iran, Iraq, Israel, Jordan, Kazakhstan, Lebanon, Palestine, Syria
T (Afghanistan, Cyprus, Iran, Iraq, Israel, Jordan, Kazakhstan, Lebanon, Palestine, Syria, Tajikistan, Turkmenistan, Turkey and Uzbekistan) (Figure 1) sourced in the Australian Grains Genebank (AGG) and currently maintained in the Plant Breeding Institute Cobbitty (PBIC). Initially, 1855 lines had been phenotyped inside the greenhouse and field in 2017 with P. hordei pathotype 5457 P+ (PBI culture No. 612), which is virulent around the ASR genes Rph1, Rph2, Rph3, Rph4, Rph6, Rph9, Rph10, Rph12, Rph19, Rph27 and avirulent for Rph5, Rph7, Rph8, Rph11, Rph13, Rph14, Rph15, Rph17, Rph18, Rph21 and Rph28. According to the initial field screening with the 1855 lines, only the resistant to moderately susceptible lines were chosen for additional testing. The lines that have been prone to lodging within the field and those with poor germination and with segregating responses to individual pathotypes in greenhouse tests had been also excluded, establishing a core set of 315 lines (Figure 2) for further MCC950 Epigenetic Reader Domain multi-pathotype tests. The passport data for the core set including origin, AGG number, taxonomy, pedigree facts (where out there) and phenotypic data for greenhouse with eight P. hordei pathotypes are supplied in Supplementary Table S1, although the data for field screening with P. hordei pathotype 5457 P+ for the years 2017, 2018 and 2019 are offered in Supplementary Table S2.Agronomy 2021, 11, 2146 Agronomy 2021, 11, x FOR PEER Review Agronomy 2021, 11, x FOR PEER REVIEW3 of 15 15 three of three ofFigure Map showing the countries of origin with the utilized in this study Figure 1. 1. Map displaying the nations of origin from the barley linesusedin this study as well as the number of lines (in brackets) Figure 1. every single country. Asia Minor and of origin of your barley linesrespectively) study plus the quantity of of lines (in brackets) barley linesused in thisare not and the number lines (in brackets) from Map showing the countries Palestine (328 and 21 lines, shown inside the map. from each country. Asia Minor and Palestine (328 and 21 lines, respectively) aren’t shown in map. from each and every nation. Asia Minor and Palestine (328 and 21 lines, respectively) are not shown in thethe map.Figure 2. Schematic diagram summarizing identification and postulation of your ASR and APR genes by means of phenotyping and genotypingSchematic diagram along with the core set (n = 315) inside the greenhouse andthe ASR and APR genes via phenotyping and Figure two. of 1855 AGG lines summarizing identification and postulation of your Plant Breeding Institute Cobbitty, NSW, Australia, fields.1855 AGG lines along with the core set (n = 315) inside the greenhouse and also the Plant Breeding Institute Cobbitty, NSW, genotyping ofAustralia, fields. Figure 2. Schematic diagram summarizing identification and postulation with the ASR and APR genes by means of phenotyping and genotyping of 1855 AGG lines as well as the core set (n = 315) inside the greenhouse plus the Plant Breeding Institute Cobbitty, NSW, Australia, fields.Agronomy 2021, 11,4 of2.two. Pathogen Isolates All of the lines with the core set have been evaluated in the seedling development stages under controlled circumstances within the greenhouse with eight Australian pathotypes (pts.) of P. hordei: 200 P- (PBI culture No. 518), 220 P+ +Rph13 (577), 253 P- (490), 5652 P+ (561), 5610 P+ (520), 5453 P- (560), 5457 P- (626) and 5457 P+ (612). All the pathotypes utilized in this study for greenhouse screening originated from annual pathogenicity surveys of P. hordei performed in DNQX disodium salt manufacturer Australia and are preserved in liquid nitrogen in the Plant Breeding Institute, Univer.