Fter TLC-DB along with the extraction parameters of SCF process. P. aeruginosa Ziritaxestat Metabolic Enzyme/Protease Sample 1 2 3 four five 6 7 eight 9 ten 11 12 13 14 15 16 17 18 19 Diameter (mm) 5.86 five.50 7.51 six.18 five.43 0.00 four.29 four.83 0.00 six.50 6.91 6.27 five.41 5.71 0.00 6.37 6.56 0.00 five.MRSA SD Diameter (mm) five.46 four.85 7.57 four.69 4.80 0.00 4.66 4.32 0.00 four.59 four.77 three.29 3.41 four.25 0.00 three.50 three.29 0.00 four.SDMPa 20 15 ten 20 15 ten 20 15 10 20 15 10 20 15 ten 20 15 10CEtOH two 2 two 2 two 2 two 2 two 1.five 1.five 1.five 1.five 1.5 1.five 1.five 1.5 1.50.72 0.56 0.85 0.64 0.42 0.00 0.71 0.29 0.00 0.42 0.20 0.33 0.47 0.80 0.00 0.78 0.55 0.00 0.0.50 0.78 0.62 0.46 0.38 0.00 0.39 0.59 0.00 0.65 0.69 0.42 0.36 0.35 0.00 0.40 0.46 0.00 0.40 40 40 60 60 60 80 80 80 40 40 40 60 60 60 80 80 80Molecules 2021, 26,4 ofTable 1. Cont. P. aeruginosa Sample 20 21 22 23 24 25 26 27 chloroform ch EtOH EtOH Vancomycin Gentamicin Diameter (mm) 6.34 five.32 five.61 4.98 0.00 3.02 2.75 0.00 0.00 0.00 0.00 eight.MRSA SD Diameter (mm) three.58 3.72 3.72 three.07 0.00 three.40 3.25 0.00 0.00 0.00 0.00 12.30SDMPa 15 10 20 15 10 20 15CEtOH 1 1 1 1 1 1 ten.82 0.41 0.47 0.61 0.00 0.23 0.48 0.00 0.00 0.00 0.00 0.0.82 0.40 0.40 0.36 0.00 0.58 0.68 0.00 0.00 0.00 0.00 0.45 -40 40 60 60 60 80 80SD = common deviation; ch = chloroform.Figure 1. (a) Detection with the antibacterial activity of clary sage SC-CO2 extracts, optimistic handle (gentamicin), and solvents against P. aeruginosa by TLC-DB. The numbering on the samples could be the similar as that shown in Table 1; (b) Detection of the antibacterial activity of clary sage SC-CO2 extracts, optimistic manage (vancomycin), and solvents against MRSA by TLC-DB. The numbering of your samples could be the similar as that shown in Table 1.Molecules 2021, 26,five ofFigure 2. Antibacterial activity of each and every clary sage extracts and gentamicin as good control on P. aeruginosa. The numbering of your samples could be the same as that shown in Table 1.Figure three. Antibacterial activity on MRSA of each and every clary sage extracts and vancomycin as constructive control. The numbering of your samples is definitely the identical as that shown in Table 1.Molecules 2021, 26,six of2.2. Response Surface Outcomes The response surface models on the extracts were plotted by using the Design Professional system. Figures 4 and five show how the antibacterial activity with the extract adjustments against bacterial strains of P. aeruginosa and MRSA in addition to the continual modify of certain parameters. It can be noticed that within the case of P. aeruginosa, the diameter on the zones of inhibition was bigger on typical, so the extracts were a lot more powerful against this sort of bacterium. The shape on the response surfaces is very comparable in the two cases studied. Because the figures show, the ideal final results were obtained at low temperatures (405 C) and moderately higher pressures (159 MPa). The least effective extracts can be prepared at 400 C and 101 MPa. Through the extraction, the plant material has to be protected from excessive heat. As a function of your MNITMT In Vivo cosolvent ratio, the shape in the figures is significantly less characteristic, from which it may be concluded that of the three setting parameters, the EtOH concentration has the least effect around the antibacterial activity. Within the case of P. aeruginosa, the optimal ratio may be among 1.6 and 1.7 , along with a additional improve in the EtOH ratio will no longer produce a substantial improvement in antibacterial activity. Nonetheless, in the case of MRSA, the best benefits might be obtained with a cosolvent ratio of two . For each bacterial strains, the worst results have been obtained at a ratio of 1.0.2 EtOH.Figure four.