0.078 lipid yield improved to 125.8 and 133.7 mg29.15 0.16 cumulative substrate11.72 g-1 utilizing 24.58 0.98 47.69 two.48 loading
0.078 lipid yield enhanced to 125.8 and 133.7 mg29.15 0.16 cumulative substrate11.72 g-1 making use of 24.58 0.98 47.69 2.48 loading of 15 1.092 1 one hundred 68.63 g-1 ), 0.37 0.52 36.37 1.92 0.086 and 20 (g four.73 respectively. Lipid eight 17.63 four.83 on one hundred g of 49.48 1.65 12.87 cobs was 1.204 developed 26.01 three.61 pretreated corn two 80 62.07 0.48 three.94 0.79 37.76 0.10 27.80 0.34 23.41 0.98 44.12 two.ten ten.33 doubled when compared with batch SSF (path A). When higher cellulase loading was applied0.069 (path 0.966 three 80 61.96 0.84 0.83 1.17 39.29 0.06 24.61 1.20 26.95 0.25 45.07 0.88 12.15 0.081 1.134 C), lipid yield and lipid 1.70 39.61fed-batch SSF as not improved (13.four g and 133.95 mg 1.106 weight in 0.70 4 80 61.81 0.14 3.97 26.42 three.39 25.23 three.16 47.16 6.69 11.90 0.079 g-1 , respectively). 0.00 0.00 34.56 1.95of lipid production (path48.24with enzyme recycle 1.078 Batch SHF tactic 1 one hundred 65.99 three.90 18.40 0.60 23.88 3.34 D) 1.60 11.52 0.077 developed only 7.two g of1.66 having a yield of 72.two 0.31 g-1 of 1.43 44.10 1.15 10.80 Regardless of 1.008 2 70 59.93 1.44 4.41 lipids 35.72 1.17 23.35 mg 24.48 pretreated corn cobs. 0.072 the enzyme savings, the cellulase loading in SHF (path D) 1.55 significantly greater than 0.994 three 70 61.43 2.15 1.82 0.46 38.55 1.26 25.96 0.12 23.30 was 46.01 three.04 ten.72 0.071 four 70 25.86 1.59 23.87 0.91 46.64 3.21 11.13 0.074 that in 61.64 fed-batch SSF course of action (path B). Acquire sn-Glycerol 3-phosphate Autophagy results suggest that fed-batch SSF lipid 1.036 the 0.26 2.02 6.65 39.29 0.XT, total cell biomass concentration (lipid-free cell biomass concentration+lipid concentration); wL, lipid content material in ce production (path B and C) enables larger lipid yield when compared with SHF with enzyme recycle biomass; L,(path concentration; YL/S, lipid yield on pretreated Gedunin Protocol lignocellulosic biomass; PrL, lipid productivity; L, lipi lipid D) and batch SSF (path A). Moreover, enzyme loading was six occasions reduced in recovery on pretreated lignocellulosic biomass (calculated according to Ivancic Santek et al. [17]). lipid employing path B compared to path C. It could be economically attractive to create thefed-batch SSF.Figure 7. Mass balance of lipid production by T. oleaginosus on hydrolysate of pretreated corn cobs Figure 7. Mass balance of cultivation approaches.T. oleaginosus on hydrolysate of pretreated corn cobs working with diverse cult utilizing different lipid production by (mL , lipid weight; YL/S , lipid yield on pretreated lignocellulosic vation strategies. (mL, lipid weight; YL/S, lipid yield on pretreated lignocellulosic biomass). biomass).4. 4. Conclusions ConclusionsProcess tactic of microbial lipidmicrobial lipid production from lignocellulosic biomass Approach tactic of production from lignocellulosic biomass affects the lipid yieldthe lipid yield and productivity. Fed-batch SSF is definitely the most effective and economical and productivity. Fed-batch SSF is the most efficient and economically feasible among the amongst the studied approaches. Further improvement of cultivation condition sible studied approaches. Additional improvement of cultivation condition, substrate feeding technique, mass transfer in culture brothculture broth hydrolysis is crucial strate feeding technique, mass transfer in and cellulase and cellulase hydrolysis is c for establishing efficient and sustainable lignocellulosic biorefinery. Recycling of adsorbed for establishing efficient and sustainable lignocellulosic biorefinery. Recycling cellulase couldsorbed cellulase could replace partSHF (up to 30 ) and lessen production and r replace par.