IP has been described in detail in humans further investigations will be required to understand the potential role of GIP in linking over-nutrition with obesity in man. Hence, provided that further studies demonstrate a role of GIP in linking over nutrition to the development of obesity in humans, and further preclinical order 86227-47-6 safety studies do not reveal toxic effects of concern, we believe that active vaccination against GIP may have the potential to be a convenient therapy for the treatment of obesity. were performed with female mice. Unless otherwise indicated 
animals were fed a high fat diet, Provimi Kliba, Switzerland), ad libitum, for the duration of the experimental period and had free access to water. Experiments were in accordance with Swiss Federal Veterinary Office guidelines. Vaccine production GIP fragment 115 including a GC linker sequence fused to the C-terminus of the GIP fragment was chemically synthesized according to standard procedures and coupled to Qb VLPs as previously described. Briefly, A solution of 1 ml of 2.0 mg/ml Qb VLPs in 20 mM Hepes, 150 mM NaCl pH 7.2 was reacted for 30 minutes with 67.2 ml of a 50 mM stock solution of SMPH in DMSO at 25uC. The reaction solution was subsequently dialyzed twice for 2 hours against 2 L of 20 mM Hepes, 150 mM NaCl, pH 7.2 at 4uC. Then 1 ml derivatized Qb VLP was reacted with 286 ml of a 10 mM peptide solution for 2 hours at 20uC in 20 mM Hepes, 150 mM NaCl, pH 7.2. The coupling reactions were then centrifuged at 13 000 rpm for 5 minutes and the supernatants were collected and dialyzed once for 2 hours and then overnight against 2 L of 20 mM Hepes, 150 mM NaCl, pH 7.2 at 4uC. The covalent chemical coupling of GIP peptides to Qb VLPs was assessed by SDS-PAGE using 12% Nu-PAGE gels. Coomassie blue stained gels of the coupling reaction demonstrated the appearance of bands with molecular weights corresponding to those predicted for GIP peptides covalently linked to QbVLPs Coupling bands corresponding to one, two, three or four peptides coupled per subunit could be identified. 15976016 Coupling efficiency was estimated, by densitometric analysis 25137254 of the Coomassie blue stained SDS-PAGE, to be between 1.62.2 GIP fragments per Qb monomer. Immunization and in vitro assays Animals were immunized subcutaneously with 100 mg or 300 mg of Qb-GIP or Qb VLPs, diluted in 200 ml PBS, at the indicated time points. GIP-specific antibody titers were determined by ELISA according to standard protocols using porcine GIP at a concentration of 2.5 mg/ml for coating. The ELISA titer is defined as the reciprocals of the serum dilution needed to reach half the maximum optical density at saturation. For competition ELISAs, a serum pool from QbGIP-immunized mice was diluted 1:2500 and incubated with increasing concentrations of porcine GIP, murine GLP-1 or murine oxyntomodulin. After overnight incubation, the amount of free antibody was quantified in a GIP-specific ELISA. For in vivo binding experiments, Qb-GIP-immunized and naive mice were challenged intravenously with 1 ng I125-GIP and 30 minutes after challenge animals were sacrificed and serum collected by cardiac puncture. Total IgG was pulled down from serum samples with protein G beads. Protein G beads associated and free radioactivity was measured and the percentage antibody bound GIP calculated. Generation of CHOK1 cells over-expressing the GIP receptor and binding studies. The human GIP receptor was Materials and Methods Animals amplified by RTPCR from a 3T3